RVG_(29)修饰脂质体对脑胶质瘤靶向性的初步研究
Preliminary study on glioma-targeting ability of RVG_(29)-modified liposome
作者：况其方;冉瑞;刘亚圆;付寒;胡关莲;何勤;
Author：
收稿日期： 年卷（期）页码：2015,30(01):-15-17
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：C6细胞;脑胶质瘤靶向性;RVG29;脂质体;薄膜分散法;细胞摄取
Key words：
基金项目：
中文摘要
目的研究一种新型配体RVG29修饰脂质体后,对体外脑胶质瘤的靶向性。方法采用有机相合成法制备DSPEPEG2000-RVG29PPP(D-RVG29)材料,按照薄膜分散法制备脂质体,通过C6细胞和Hela细胞的细胞摄取考察D-RVG29修饰脂质体后,对体外脑胶质瘤的靶向性。结果在脂质体处方中加入DSPE-PEG2000-OME(D-OME)可使D-RVG29修饰脂质体具有更好的粒径和分布范围,C6细胞对D-RVG29修饰脂质体的摄取强于未修饰D-RVG29的脂质体,Hela细胞对两种脂质体的摄取强度无明显区别。结论 D-OME可以提高D-RVG29修饰脂质体的稳定性,D-RVG29修饰脂质体具有体外脑胶质瘤细胞的靶向性。
参考文献
[1]Cheng G.Target delivery of a gene into the brain using the RVG29-oligoarginine peptide[J].Biomaterials,2012,33:3456-3463.
[2]Mauro R.A model of the rabies virus clycoprotein active site[J].Biopolymers,1993,33:961-969.
[3]Qianyu Z.A p H-responsive a-helical cell penetrating peptide-mediated liposomal delivery system[J].Biomaterials,2013,34:7980-7993.
[4]Xu H.Esterase catalyzed de PEGylation of p H-sensitive vesicles modified with cleavable PEG-lipid derivatives[J].J Controlled Release,2008,130:38-245.
[5]Do WH.A brain-targeted rabies virus glycoprotein-disulfide linked PEI nanocarrier for delivery of neurogenic microRNA[J].Biomaterials,2011,32:4968-4975.
[6]Sejin S.RVG peptide tethered bioreducible polyethylenimine for gene delivery to brain[J].J Controlled Release,2011,155:18-25.
[7]Kumar P.Transvascular delivery of small interfering RNA to the central nervous system[J].Nature,2007,448:39-43.
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