银杏叶内酯N对谷氨酸损伤PC12细胞的保护作用
Protective effects of ginkgolide N on the PC12 cells injury induced by glutamate
作者：马舒伟;张现涛;刘红霞;韩光程;
Author：
收稿日期： 年卷（期）页码：2011,26(02):-138-140
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：银杏叶内酯N;PC12细胞;谷氨酸损伤;脑缺血
Key words：
基金项目：粤港关键领域重点突破项目(2009498C15)
中文摘要
目的观察银杏叶内酯N(GN)对谷氨酸诱导PC12细胞损伤的保护作用及与银杏叶内酯B(GB)的药效比较。方法采用谷氨酸诱导的PC12细胞损伤作为脑缺血损伤的体外研究模型,采用MTT比色法和乳酸脱氢酶(LDH)活力检测法,观察GN对谷氨酸致PC12细胞损伤的保护作用。结果 GN能明显改善谷氨酸诱导损伤的PC12细胞的活力,并能使损伤细胞上清液中的LDH含量明显减少,活性大于GB。结论 GN可减轻兴奋性氨基酸所致的神经元损伤,对神经元具有保护作用,药理活性优于GB。
参考文献
[1]Cho JH,Sung JH,Cho EH,et al.Gingko biloba extract(EGb761)prevents ischemic brain injury by activation of the Akt sig-naling pathway[J].Am J Chin Med,2009,37(3):547-555.
[2]Wu X,Qian Z,Ke Y,et al.Ginkgolide B preconditioning protects neurons against ischaemia-induced apoptosis[J].J Cell MolMed,2009,13(12):4474-4483.
[3]Hsuuw YD,Kuo TF,Lee KH,et al.Ginkgolide B induces apopto-sis via activation of JNK and p21-activated protein kinase 2 inmouse embryonic stem cells[J].Ann N Y Acad Sci,2009,1171:501-508.
[4]Bone KM.Potential interaction of Ginkgo biloba leaf with anti-platelet or anticoagulant drugs:what is the evidence[J].MolNutr Food Res,2008,52(7):764-671.
[5]ZHANG XT,LI Y,ZHANG LH,et al.A new ginkgolide fromGinkgo biloba[J].J China Pharmac University,2009,40(4):306-309.
[6]Singh G,Siddiqui MA,Khanna VK,et al.Oxygen glucose depri-vation model of cerebral stroke in PC-12 cells:glucose as alimiting factor[J].Toxicol Mech Methods,2009,19(2):154-160.
[7]Pinar A,Servet K,Murat O,et al.Pregnenolone protects the PC-12 cell line against amyloid beta peptide toxicity but its sulfateester does not[J].Chemico-Biological Interactions,2009,177(1):65-70.
【关闭】
