罗非鱼鱼鳞胶原蛋白多肽对小鼠成骨细胞增殖与Ⅰ型胶原蛋白分泌的影响
Effects of collagen polypeptide of tilapia fish scale on proliferation of mouse osteoblastic cell and type Ⅰ collagen secretion
作者:宋芹;颜军;郭晓强;姚倩;郁小兵;
Author:
收稿日期: 年卷(期)页码:2011,26(02):-130-132
期刊名称:华西药学杂志
Journal Name:WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字:罗非鱼;鱼鳞胶原蛋白多肽;MC3T3-E1细胞;增殖;Ⅰ型胶原蛋白分泌;mRNA表达
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基金项目:
中文摘要
目的研究不同分子量的罗非鱼鱼鳞胶原蛋白多肽对体外培养新生小鼠颅骨MC3T3-E1细胞增殖与Ⅰ型胶原蛋白分泌及Ⅰ型胶原蛋白mRNA表达的影响。方法采用WST-8法测定胶原蛋白多肽对小鼠成骨细胞株MC3T3-E1细胞增殖的影响,ELISA法检测胶原蛋白多肽对MC3T3-E1细胞Ⅰ型胶原蛋白分泌的影响,采用real-time PCR分析不同分子量的胶原蛋白多肽对Ⅰ型胶原蛋白mRNA表达的影响。结果胶原蛋白多肽能促进MC3T3-E1细胞增殖(P
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