SKLB010对MRL/lpr小鼠尿蛋白和单核细胞趋化蛋白-1表达的影响
Effect of SKLB010 on albuminuria and monocytes chemotactic protein-1 expression in MRL/lpr mice
作者：宋丽芳;王飞;胡小红;童擎一;李怡;吴晓华;
Author：
收稿日期： 年卷（期）页码：2011,26(02):-124-126
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：单核细胞趋化蛋白-1;尿蛋白;CD68;SKLB010
Key words：
基金项目：国家“重大新药创制”科技重大专项项目(2009ZX09102-045);;
教育部留学回国人员科研启动基金(JS20081007506765)
中文摘要
目的探讨小分子药物SKLB010对自发性狼疮小鼠肾脏中单核细胞趋化蛋白-1(MCP-1)表达的影响,研究SKLB010对MRL/lpr狼疮小鼠的治疗作用,并初步探讨其作用机制。方法将20只8周龄狼疮小鼠,随机分为SKLB010治疗组与模型组。治疗组经ig口服50 mg·kg-1.d-1 SKLB010后,观察小鼠的尿蛋白、肾功能与肾脏病理的改变。运用免疫组织化学,检测MRL/lpr狼疮小鼠肾组织中MCP-1、CD68的表达。结果模型组狼疮小鼠的尿蛋白水平上调,肾小球基底膜增厚,系膜基质增多,肾组织中MCP-1表达上调,CD68阳性细胞数明显增多。与模型组相比,治疗组狼疮小鼠的尿蛋白水平下降,肾组织中MCP-1表达减少,CD68阳性细胞数减少。结论 SKLB010对MRL/lpr小鼠的自发狼疮肾炎具有治疗作用,其机制可能通过抑制肾组织中MCP-1的表达,减少肾组织单核细胞的浸润,从而延缓狼疮性肾炎的病理损害。
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