利福平固体脂质纳米粒的体外细胞摄取研究
Preparation of Rifampicin-solid lipid nanoparticles and evaluation of their cell specificity
作者：串俊兰;龚涛;孙逊;林箐;张志荣;
Author：
收稿日期： 年卷（期）页码：2011,26(03):-223-225
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：利福平;固体脂质纳米粒;细胞毒性;高效液相色谱法;巨噬细胞
Key words：
基金项目：国家自然科学基金(批准号:30873165)
中文摘要
目的研究利福平固体脂质纳米粒的制备方法和体外细胞摄取。方法采用薄膜分散法制备利福平固体脂质纳米粒(RFP-SLNs);用扫描电镜观察其形态,激光粒度仪测定其粒径大小和Zeta电位;用MTT法测定其对RAW 264.7、A549细胞的毒性,HPLC法测定其在这两种细胞中的摄取量。结果 RFP-SLNs的外观形态圆整,为类球形,粒径为812.2 nm,Zeta电位为-38.4 mV;当RFP-SLNs的浓度不大于20.0μg·mL-1(RFP的含量)时,两种细胞存活率均大于80%。RAW 264.7、A549两种细胞对RFP-SLNs的摄取量分别为691.7、319.2 ng·mg-1。结论 RFP-SLNs能被RAW 264.7特异性摄取,具有体外的巨噬细胞靶向性。
参考文献
[1]Sumio C,Tomoharu T,Toshiobu S,et al.Influence of particle sizeon drug delivery to rat alveolar macrophages following pulmonaryadministration of ciprofloxacin incorporated into liposomes[J].JDrug Targeting,2006,14(8):557-566.
[2]Panchagnula R,Sood A,Sharda N,et al.Determination of rifampi-cin and its main metabolite in plasma and urine in presence ofpyrazinamide and isoniazid by HPLC method[J].J Pharmaceuti-cal and Biomedical Analysis,1999:1013-1020.
[3]李艳贞,刘洁,孙逊,等.新型肺部吸入微球的制备及性质的研究[J].华西药学杂志,2009,24(6):573-575.
[4]范彩霞,高文慧,陈志良,等.体外评价羧甲基壳聚糖超顺磁氧化铁纳米粒的细胞毒性和巨噬细胞的摄取[J].华西药学杂志,2010,25(3):290-293.
[5]Sumio C,Tomoharu T,Toshinobu S,et al.Uptake characteristicsof liposomes by rat alveolar macrophages:influence of particlesize and surface mannose modification[J].J Pharmacy and Phar-macology,2007,59:75-80.
【关闭】
