HPLC同时测定慢性阻塞性肺病患者血浆中的茶碱和1,3-二甲基尿酸
Simultaneous determination of theophylline and 1,3-dimethyluric acid in the patients plasma with chronic obstructive pulmonary disease by HPLC
作者：康迎波;陈新;宋帅;朱哲;王黎青;张忠义;
Author：
收稿日期： 年卷（期）页码：2015,30(03):-328-330
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：茶碱;1,3-二甲基尿酸;慢性阻塞性肺病;血药浓度;高效液相色谱;清除率;同时测定
Key words：
基金项目：国家自然科学基金资助项目(批准号:81302846);;
广东省科技计划项目(2013B022000072)
中文摘要
目的采用HPLC法同时测定慢性阻塞性肺病(COPD)患者血浆中的茶碱及其代谢物1,3-二甲基尿酸(1,3-DMU)。方法色谱柱为Hypersil BDS C18柱(250 mm×4.6 mm,5μm),流动相为乙腈-水(7∶93),流速1 m L·min-1,检测波长280 nm。43名COPD患者连续服用茶碱(100 mg,bid)两周后,测定茶碱及1,3-DMU的血药浓度。结果茶碱、1,3-DMU的线性范围分别为0.20~10.00μg·m L-1(r=0.9998)、0.05~2.00μg·m L-1(r=0.9996);日内、日间RSD均≤12.91%,提取回收率分别为77.52%~79.22%、71.69%~74.02%;患者的平均茶碱血药浓度为3.57±1.59μg·m L-1,茶碱和1,3-DMU的浓度呈强相关(r=0.672),1,3-DMU/茶碱为0.064±0.024。结论所用方法简便、快速、准确,可用于COPD患者茶碱血药浓度的监测,1,3-DMU与茶碱浓度的比值可评估不同个体对茶碱清除率的差异。
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