HPLC测定伏康树籽中的柳叶水甘草碱
Determination of tabersonine in the seeds of Voacanga africana by HPLC
作者：白珂珂;苏艳红;陈晓春;姚晨;蔡元丽;李晖;
Author：
收稿日期： 年卷（期）页码：2011,26(05):-492-493
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：高效液相色谱法;柳叶水甘草碱;伏康树籽;含量
Key words：
基金项目：
中文摘要
目的采用HPLC测定伏康树籽中柳叶水甘草碱的含量。方法色谱柱为Agilent Extend-C18柱(150 mm×4.6 mm,5μm);柱温30℃;流动相为甲醇-0.1 mol.L-1醋酸铵(pH6)(70∶30);检测波长290 nm;流速1.0 mL.min-1;进样量20μL。结果柳叶水甘草碱的进样量0.1708～5.4656μg与峰面积具有良好的线性关系,回归方程为:Y=7.2697×103X-9.1125(r=0.9995)。伏康树籽中柳叶水甘草碱的含量为2.65%。结论所用方法操作简便、快捷、结果准确。
参考文献
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