UPLC-MS/MS法测定人血清中的神经内分泌肿瘤标志物5-羟基吲哚乙酸
Determination of 5-hydroxyindoleacetic acid in serum as neuroendocrine tumor marker by UPLC-MS / MS
作者：段虹飞;李巧艳;董玲芳;赵宁民;秦玉花;赵红卫;张伟;
Author：
收稿日期： 年卷（期）页码：2015,30(04):-481-483
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：超高效液相色谱串联质谱法;5-羟基吲哚乙酸;神经内分泌肿瘤;肿瘤标志物;血清;诊断
Key words：
基金项目：河南省医学科技攻关计划项目(201203134)
中文摘要
目的采用超高效液相色谱串联质谱法测定人血清中5-羟基吲哚乙酸(5-HIAA)的含量。方法采用Waters Acquity UPLC BEH C18色谱柱(50 mm×2.1 mm,1.7μm),流动相为2.5 mmol·L-1乙酸铵水溶液(含0.02%甲酸)-甲醇(68∶32),流速0.2 m L·min-1,进样量3μL,柱温35℃,样品室温度6℃;采用电喷雾正离子模式电离,5-HIAA和甲氨蝶呤内标的监测离子分别为m/z 192.2→146.0、m/z 455.3→308.2。结果 5-HIAA的线性范围为0.0100~10.02μmol·L-1,平均回收率为92.1%~96.5%,批内、批间RSD均小于15%。结论所用方法的线性范围宽、分析时间短,可用于神经内分泌肿瘤的诊断及进展监测。
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