HPLC手性固定相法拆分异丙肾上腺素对映体
Enantioseparation of isoprenalinum derivatives on chiral stationary phase by HPLC
作者：曹雪玲;李鑫;任红;
Author：
收稿日期： 年卷（期）页码：2015,30(05):-601-603
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：手性固定相;异丙肾上腺素;衍生化;氯甲酸苄酯;手性拆分;高效液相色谱
Key words：
基金项目：吉林省教育厅“十二五”科学技术研究项目[第432号]
中文摘要
目的采用手性固定相高效液相色谱法拆分异丙肾上腺素对映体。方法以氯甲酸苄酯作为非手性衍生化试剂,与异丙肾上腺素进行衍生化反应,通过Chiral PAKRAD-RH手性色谱柱分离。检测波长选择为226 nm,流动相为甲醇-水-四氢呋喃(150∶20∶7),流速0.5 m L·min-1,柱温30℃。结果最大分离度达到1.61,成功折分了异丙肾上腺素对映体。结论所用方法具有操作简单、反应条件温和、分离度高等特点,可为此类药物对映体的工业化分离和制备提供新的方法。
参考文献
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