大黄水煎液中大黄酸在急性肝损伤大鼠体内的药动学研究
Study on pharmacokinetics of rhein in acute hepatic injury rats after oral administration of rhubarb extracts
作者：张艳;徐佑东;胡樱凡;王平;孟宪丽;
Author：
收稿日期： 年卷（期）页码：2015,30(05):-571-574
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：大黄;大黄酸;急性肝损伤;D-氨基半乳糖;高效液相色谱;荧光法;药动学;房室模型
Key words：
基金项目：国家自然科学基金资助项目(批准号:81473419);;
四川省教育厅自然科学重点项目(11ZA061);;
四川省科技厅省青年科技创新研究团队专项计划项目(2014TD0007)
中文摘要
目的比较大黄水煎液中大黄酸在正常与急性肝损伤大鼠体内的药动学差异。方法采用一次性ip D-氨基半乳糖制备大鼠急性肝损伤模型,单次ig大黄水煎液1.54 g·kg-1后,于不同时间点取血,分离血浆,用HPLC-荧光法测定大黄酸的血药浓度,以Kinetica软件计算药动学参数。结果药动学过程均符合二室模型。正常组雌鼠体内大黄酸Cmax和AUC均显著高于雄鼠。急性肝损伤组雄鼠的Kel显著增加,MRT显著缩短,AUC显著降低。结论大黄酸在雌性大鼠体内的生物利用度高于雄性;大黄酸在肝损伤大鼠体内消除较快,吸收量较小,其动力学改变可能是由于肝脏对大黄游离蒽醌存在明显代谢或转化作用。
参考文献
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