rhGH和IFN-γ对肝硬化大鼠肝组织中蛋白合成代谢的影响
The effects of rhGH and IFN-γ on the liver protein synthesis and metabolism in the liver cirrhosis rats in vivo
作者：陈拥华;杜冰清;郑振江;刘续宝;陆燕蓉;麦刚;
Author：
收稿日期： 年卷（期）页码：2011,26(06):-560-563
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：重组生长激素;重组干扰素-γ;肝硬化;白蛋白;代谢
Key words：
基金项目：
中文摘要
目的了解重组生长激素(rhGH)及γ-干扰素(IFN-γ)对肝硬化蛋白合成代谢的影响。方法采用Proctor改良法,建立CCl4致大鼠肝硬化模型,合格模型随机分为空白对照组(NS,1 mL.d-1,sc)、阳性对照组(马洛替酯100 mg.kg-1.d-1,ig)、rhGH组(rhGH 0.8 IU.kg-1.d-1,sc)、rhGH+IFN-γ组(rhGH 0.8 IU.kg-1.d-1,sc),自第14天开始同时sc IFN-γ2×104IU.d-1,分别于实验第7、14、28天处死实验大鼠。观察大鼠体重的变化、尿氮排除量,检测肝脏白蛋白的mRNA,用凝胶扫描仪扫描测定各电泳带的光密度值。结果 rhGH组体重增加量高于空白对照组及阳性对照组(P0.05)。结论外源性rhGH可促进肝硬化大鼠的肝细胞中白蛋白基因表达、mRNA和白蛋白的合成,提高血中白蛋白水平,增加体重,减少尿氮排出,促负氮平衡的恢复。加用IFN-γ后不影响rhGH促进肝硬化大鼠肝脏白蛋白基因的表达。
参考文献
[1]Blaas L,Kornfeld JW,Schramek D,et al.Disruption of the growthhormone-signal transducer and activator of transcription 5-in-sulinlike growth factor 1 axis severely aggravates liver fibrosis in amouse model of cholestasis[J].Hepatology,2010,51(4):1319-1326.
[2]Chen S,Wang HT,Yang B,et al.Protective effects of recombinanthuman growth hormone on cirrhotic rats[J].World J Gastroen-terol,2004,10(19):2894-2897.
[3]Li N,Zhou L,Zhang B,et al.Recombinant human growth hor-mone increases albumin and prolongs survival in patients withchronic liver failure:A pilot open,randomized,and controlledclinical trial[J].Digest Liver Dis,2008,40:554-559.
[4]Proctor E,Chatamra K.Control induction of cirrhosis in the rat[J].Br J Exp Path,1983,64(3):320.
[5]严律南,曾勇.重组人生长激素对肝切除术后肝脏再生作用的临床研究[J].中国实用外科杂志,1999,19(l):21-23.
[6]Raguso CA,Genton L,Kyle U,et al.Management of catabolism inmetabolically stressed patients:a literature survey about growthhormone application[J].Curr Opin Clin Nutr Metab Care,2001,4(4):313-320.
[7]Assy N,Pruzansky Y,Gaitini D,et al.Growth hormone-stimula-ted IGF-1 generation in cirrhosis reflects hepatocellular dysfunc-tion[J].J Hepatol,2008,49(1):34-42.
[8]Weng HL,Wang BE,Jia JD,et al.Effect of interferon-gamma onhepatic fibrosis in chronic hepatitis B virus infection:A random-ized controlled study[J].Clin Gastroenterol Hm,2005,3(8):819-828.
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