牛蒡子苷对糖尿病肾病大鼠肾小球滤过屏障损伤的保护作用
The protection effect of arctiin on filtration barrier injury of kidney glomerulus in diabetic nephropathy rats
作者：刘冬恋;莫正纪;马松涛;牛锐;刘睿斌;
Author：
收稿日期： 年卷（期）页码：2011,26(06):-536-539
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：糖尿病肾病;牛蒡子苷;Nephrin;Podocin;乙酰肝素酶
Key words：
基金项目：国家“十一五”重大新药创制科技重大专项课题(2008ZX09101-Z-019)
中文摘要
目的探讨牛蒡子苷对糖尿病肾病(DN)大鼠肾小球滤过屏障中nephrin、podocin和乙酰肝素酶(HPSE)表达的影响。方法将SD大鼠制备成DN实验模型,随机分为模型组、牛蒡子苷小、大剂量组(ig 40、60 mg.kg-1.d-1)正常对照组。8周后观察各组血糖、24 h尿蛋白定量及肾脏指数,光镜下观察肾组织的病理改变,采用免疫组化和Real time-PCR检测肾皮质中nephrin、podocin、HPSE蛋白和mRNA的表达。结果牛蒡子苷可降低DN大鼠尿蛋白的排泄,降低肾脏指数,改善肾脏病理,使nephrin、podocin蛋白和mRNA的表达增加,HPSE蛋白的表达降低。结论牛蒡子苷对DN大鼠具有一定的肾脏保护作用,其作用机制可能与调控肾小球滤过屏障中相关蛋白的表达有关。
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