生物素对高糖状态下大鼠胰岛β细胞功能的影响
The effect of biotin on the function of rat islet β cell cultured with high glucose
作者：朱晓波;闫智宏;徐志伟;宋桂芹;王振辉;
Author：
收稿日期： 年卷（期）页码：2010,25(01):-29-31
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：生物素;葡萄糖;胰岛;胰岛素;前胰岛素源mRNA
Key words：
基金项目：河北省科学技术研究与发展计划项目(批准号:07276101D-4)
中文摘要
目的探讨生物素对高糖损伤胰岛β细胞功能的影响。方法采用Dextran密度梯度离心法分离纯化大鼠胰岛,在含5.5mmol·L-1葡萄糖、5.5mmol·L-1葡萄糖+1μmol·L-1生物素、含27.0mmol·L-1葡萄糖及27.0mmol·L-1葡萄糖+1μmol·L-1生物素的RPMI1640培养基中培养48h,分析3.3、27.0mmol·L-1葡萄糖刺激的胰岛素分泌及胰岛细胞中胰岛素的含量;提取总mRNA,RT-PCR扩增前胰岛素源基因,电泳并进行灰度分析。结果在5.5mmol·L-1葡萄糖培养条件下,生物素增加高糖诱导的胰岛素释放及胰岛细胞的胰岛素含量,前胰岛素源mRNA也增加;在27.0mmol·L-1葡萄糖培养48h的胰岛中,胰岛细胞中的胰岛素浓度降低,高糖诱导的胰岛素释放及前胰岛素源mRNA显著减少;生物素的共培养,部分提高了高糖诱导的胰岛素释放及前胰岛素源mRNA含量。结论生物素通过促进胰岛素的合成显著改善了高糖损伤胰岛β细胞的功能紊乱。
参考文献
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