天然酮化合物抗柯萨奇病毒B_5的作用
Antiviral activity of natural xanthone against Coxackie virus B_5 in vitro
作者：刘钊;刘婧;刘媛媛;杨占秋;杨光忠;
Author：
收稿日期： 年卷（期）页码：2010,25(01):-19-22
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：酮;柯萨奇病毒;抗病毒作用
Key words：
基金项目：中南民族大学自然科学基金资助项目(批准号:YZQ05011)
中文摘要
目的研究天然酮类化合物抗柯萨奇病毒B5(CVB5)的作用及其机理。方法通过改变给药途径,观察病毒引起的细胞病变效应(CPE),MTT法检测细胞活性,评价酮化合物在Hep-2细胞培养中的抗CVB5作用。结果酮具有抗CVB5的活性,对Hep-2细胞的TC50为54.44μg·mL-1,其EC50分别为26.378、25.813、25.405μg·mL-1,治疗指数(TI)分别为2.064、2.109、2.143;在32μg·mL-1时,对CVB5的抑制率达50%。结论酮化合物通过直接杀灭病毒、阻止病毒的吸附、抑制病毒的生物合成而发挥其抗CVB5的作用;但需对结构做进一步的修饰,以降低其毒性。
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