HPLC测定左乙拉西坦中的右旋异构体
Determination of dextro isomer in Levetiracetam by HPLC
作者：崔蕊;李文莉;崔超;张丹;
Author：
收稿日期： 年卷（期）页码：2010,25(05):-593-594
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：左乙拉西坦;光学异构体;右旋体;高效液相色谱法
Key words：
基金项目：
中文摘要
目的采用HPLC法测定左乙拉西坦中的右旋体。方法采用ChiralcelOD-H色谱柱(250mm×4.6mm,5μm);流动相为正己烷-异丙醇-三氟乙酸(90∶10∶0.1);流速1.0mL·min-1;检测波长218nm。结果左乙拉西坦与其右旋体得到了基线分离,其右旋体0.4～3.6×103μg·mL-1与峰面积的线性关系良好(r=0.9998),最低检测限为4ng,定量限为8ng。结论所建方法准确、简便、灵敏,适用于左乙拉西坦的质量控制。
参考文献
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