碳纳米管靶向抗肿瘤药物载体的制备及初步分析
Preparation and preliminary evaluation of targeted carbon nanotube antitumor drug transporter
作者：何维;王剑;
Author：
收稿日期： 年卷（期）页码：2010,25(06):-664-668
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：碳纳米管;阿霉素;靶向;药物传递系统;抗癌活性
Key words：
基金项目：口腔疾病研究国家重点实验室自由申请项目(SKLODPD002);;
四川大学青年基金(批准号:2008076)
中文摘要
目的制备单壁碳纳米管(SWNTs)靶向抗肿瘤药物载体,初步分析其药学特性和细胞学特性。方法功能化处理SWNTs后,在其表面负载各种生物活性分子,包括表面改性分子叶酸-聚乙二醇(PEG-FA)、抗肿瘤化疗药物阿霉素(DOX)以及荧光标记分子异硫氰酸荧光素(FITC),考察各生物分子负载情况及该药物载体的体外细胞学特性。结果酸处理及PEG-FA功能化后的SWNT能稳定分散于水中,长度为200~500 nm,浓度最高可达50μg.mL-1;DOX及FITC能在SWNTs表面稳定结合,最佳结合值pH7;药物载体能通过叶酸分子的靶向介导进入肿瘤细胞,较游离DOX有更强的体外抑癌效能。结论碳纳米管靶向抗肿瘤药物载体能靶向作用于肿瘤细胞,性状稳定,载药量可控,增加了化疗药物的抗癌活性。
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