PELA疫苗微球载体的正交优化工艺研究
TECHNIC STUDY ON THE PELA MICROSPHERE VEHICLE LOADED VACCINE BY ORTHOGONAL EXPERIMENTS
作者：任建敏,邹全明,彭承琳,田文标,张玮
Author：
收稿日期： 年卷（期）页码：2002,(01):-7-9
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：正交优化;疫苗微球;牛白蛋白
Key words：
基金项目：
中文摘要
目的 利用正交设计的数学方法进行实验设计 ,以牛白蛋白为包裹模型 ,寻求制备不同疫苗微球载体的最佳条件。方法 采用W /O/W法制备PELA微球 ,考察不同因素对疫苗微球平均粒径、成球率与蛋白包裹率 (三指标 )的影响。结果 五因素对疫苗微球成球性影响的主次顺序为PVA浓度 >搅拌速度 >PELA浓度 >油外 /外水相体积比 >内水相体积 ;而对粒径与蛋白包裹率的顺序则为PELA浓度 >PVA浓度 >搅拌速度 >油相 /外水相体积比 >内水相体积和PELA浓度 >PVA浓度 >搅拌速度 >内水相体积 >油相 /外水相体积比。结论 控制工艺条件 ,可制备适于不同需求的疫苗微球载体
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