苯酚-硫酸比色法测定远志及地上部分多糖的含量
Determining the polysaccharides from Polygala tenuifolia by Phenol-sulphate colorimetry
作者:裴瑾,万德光,杨林
Author:
收稿日期: 年卷(期)页码:2005,(04):-337-339
期刊名称:华西药学杂志
Journal Name:WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字:远志;多糖;紫外分光光度法
Key words:
基金项目:国家科技部“863”攻关项目,项目编号:2001BA701A55-44
中文摘要
目的建立远志多糖的含量测定方法。方法采用苯酚-硫酸比色法。结果葡萄糖在0.025~0.125mg.ml-1线性范围内平均回收率为96.67%,RSD=3.78%(n=6),远志根多糖含量为4.84%,地上部分为6.86%。结论所用方法快速、准确,便于实际应用,远志地上部分具有综合利用价值。
参考文献
[1]吴寿金,赵泰,秦永祺.现代中草药成分化学[M].北京:中国医药科技出版社,2002.19
[2]泷浦,本田.远志の糖成分につぃて[J].药学杂志,1993,47(3):1223
[3] Miyase Toshio, Ueno Akira. Sucrose derivatives from the roots ofPolygala tenuifolia[J].生药学杂志,1993,47(3):267
[4] Miyase Toshio, Iwata Yukiko, Uend Akira. Tenuifolises A- F,oligosaccharide mullt - esters from the roots ofPolygala tenuifolia[J]. Chem Pharm Bull,1991,39(11):3082
[5] Miyase Toshio,Iwata Yukiko, Uend Akira. Tenuifolises G - P,oligosaccharide mullt - esters from the roots ofPolygala tenuifolia[J]. Chem Pharm Bull,1992,40(10):2741
[6] Wang Sha Sha,Kozuka Koji, Komotsu Keni- chi,et al. Pharmaco-logical properties of galenical properations (ⅩⅧ): Pharmacokineticsof active compounds of Polygalae Radix[J]. J Trad Med,1994,11:168
[7] Wang ShaSha, Saito Ken-ichi, Owada Ejji,et al.Pharmacologicalproperties of galenical properations (ⅩⅩ): Screening of naturalproducts in Polygalae Radix[J].J Trad Med,1995,12:102
[8]饭冢进.远志的脑保护活性成分[J].国外医学.中医中药分册,1995,17(2):44
[9]池谷幸信.远志中吨酮糖甙及配基糖的研究[J].国外医学.中医中药分册,1995,17(2):44
【关闭】