壳聚糖/聚乙烯醇微球吸附脲酶的特性及其动力学
Activity and kinetic properties of urease adsorbed onto chitosan/PVA microspheres
作者：任建敏;吴四维;余纯丽;
Author：
收稿日期： 年卷（期）页码：2006,(04):-328-330
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：脲酶;壳聚糖/PVA;微球;吸附
Key words：
基金项目：
中文摘要
目的制备壳聚糖/聚乙烯醇(PVA)微球脲酶,探讨其最适温度、最适pH、热稳定性及其动力学。方法用微乳法制备壳聚糖/PVA微球,采用电子扫描电镜表征其形态;用吸附法获得壳聚糖/PVA微球脲酶;运用Berthlot测定其活力。结果壳聚糖/PVA微球脲酶酶活性保持率是原酶的91.93%,最适温度为47℃,最适pH6.5,热稳定性明显高于溶液脲酶;壳聚糖/PVA微球脲酶的动力学参数KM=11.8 mmo.lL-1,Vm ax=37.1μmo.lm in-.1mg-1。结论壳聚糖/PVA微球脲酶可制备成用于尿毒症患者的口服制剂。
参考文献
[1]L iang ZP,Feng YQ,L iang ZY,et a.l Adsorption of urea n itrogenonto ch itosan coated d ialdehyde cellu lose under b iocatalysis ofimmob ilized urease:Equ ilibrium and k inetic[J].B iochem icalEngineering J,2005,24(1):65-67.
[2]Martino AD,S ittinger M,R isbud MV.Ch itosan:A versatile b io-polym er for orthopaed ic tissue-engineering[J].B iomaterials,2005,30(26):5983.
[3]W an Ngah W S,Kamari A,Koay YJ.Equ ilibrium and k ineticsstud ies of adsorption of copper(II)on ch itosan and ch itosan/PVA beads[J].Int J B iologicalMacromolecu les,2004,34(3):155-161.
[4]Chaudhari PS,Gokarna A,Ku lkarn iM,et a.l Porous silicon as anentrapp ing matrix for the immob ilization of urease[J].Sensorsand Actuators B:Chem ical,2005,107(1):258-263.
[5]X i FN,W u JM,Jia ZS,et al.Preparation and characterization oftrypsin immob ilized on silica gel supported macroporous ch itosanbead[J].Process B ioch,2005,40(8):2833-2840.
[6]God jevargova T,VelikovaM,Vasileva N,et a.lM etallochelate im-mob ilization of urease on to amorphous S iO2[J].Process B io-chem istry,2005,40(9):3045-3049.
[7]Bayramof lu G,Yalcin E,Ar ca MY.Immob ilization of urease viaadsorption onto l-h istid ine-N i(II)complexed poly(HEMA-MAH)m icrospheres:Preparation and characterization[J].Process B iochem istry,2005,40(11):3505-3513.
【关闭】
