人抗菌肽FALL-39突变分子的特性和抗菌功能的比较
Comparing the protein characteristics and antibacterial activity of antibacterial peptide FALL-39 and its mutant peptides
作者：杨云霞;朱玲;王伯瑶;冯云;
Author：
收稿日期： 年卷（期）页码：2006,(05):-422-424
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：人抗菌肽FALL-39;蛋白特性;抗菌功能;突变分子
Key words：
基金项目：CMB(98-681)基金资助
中文摘要
目的对人抗菌肽FALL-39不同突变分子蛋白的特性及抗菌功能进行初步的比较。方法应用AU-PAGE电泳、RP-HPLC、Om iga蛋白分析软件,比较FALL-39及其突变蛋白FALL-39-Lys24、FALL-39-Lys32、FALL-39-Lys24′32的特性;测定M IC、MEC和MBC值,并比较抗菌作用。结果突变蛋白AU-PAGE电泳迁移速率较快,RP-HPLC出峰时间未见改变,蛋白的亲疏水性、α螺旋结构、抗原性等蛋白特性无明显改变。M IC、MEC和MBC值表明FALL-39突变肽对大肠杆菌和绿脓杆菌的抗菌活性有所增强。结论突变蛋白在增加正电荷的同时,虽未改变蛋白的特性,但抗菌活性增强。
参考文献
[1]Lehrer R I,Ganz T.Cathelicid ins:a fam ily of endogenous anti-m icrob ial peptides[J].Curr Opin Hematol,2002,9(1):18-22.
[2]Gudmundsson GH,Agerberth B,Adeberg J,et a.l The humangene FALL-39 and processing of the cathlin precursor to the an-tim icrob ial peptide LL-37 in granu locytes[J].Eur J b iochem,1996,238(2):325-332.
[3]Gudmundsson GH,Magnusson KP,Chowdhary BP,et a.l Struc-ture of the gene for porcine peptide antib iotic PR-39,a cathelingene fam ily m ember:comparative mapp ing of the locus for thehuman peptide antib iotic FALL-39[J].Proc Natl Acad SciUSA,1995,92:7085-7089.
[4]Yang YX,Feng Yun,W ang BY,et a.l PCR-based site-spe-cific mutagensis of FALL-39 and its b iologic activities[J].ActaPharmacological S in ica,2004,25(2):239-245.
[5]O ren Z,Lerman JC,Gudmundsson GH,et a.l Structure and or-gan ization of the human antim icrob ial peptide LL37 in phospho-lip id m embranes:relevance to the molecu lar basis for its non-cell-selective activity[J].B iochem J,1999,341:501-513.
[6]奥斯伯F.精编分子生物学实验指南[M].北京:科学出版社.1998.388-392.
[7]Mark AF,Marek AK.A novel peptide-IgG con jugate,CAP18106-138-IgG,that b ind ing and neutralizes endotoxin and k illsGram-negative bacteria[J].Infectious D isease,1997,175:621.
[8]Zhao CQ,Nguyen T,Boo LM,et a.l RL-37,an alpha-heli-cal antim icrob ial peptide of the rhesus monkey[J].Antim icrobAgents Chemother,2001,45(10):2695-2702.
【关闭】
