DNA阵列芯片上的DNA二聚体并行热力学研究及其在SNP基因分析中的应用
Parallel thermodynamic analysis of duplexes on oligo DNA microarray and its important usage in SNP analysis
作者：李征,朱兵,高晓莲
Author：
收稿日期： 年卷（期）页码：2005,(05):-396-399
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：DNA;DNA阵列芯片;SNP分析;解链温度;热力学研究;并行
Key words：
基金项目：国家留学基金委资助
中文摘要
目的用DNA阵列芯片进行SNP的探测。方法根据DNA二聚体的热力学性质,在DNA阵列芯片上测定其解链曲线,用数学的方法获得其解链温度(Tm)值,再根据Tm值进行SNP分析。结果用特殊设计的寡聚DNA阵列芯片进行并行热力学研究,测得其DNA二聚体的解链曲线,计算出Tm值,并进行了SNP分析。结论可用DNA阵列芯片成功地进行高通量的SNP基因分析。
参考文献
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