壳聚糖-海藻酸钠幽门螺杆菌全菌蛋白微球的制备及表征
Preparation and character of microspheres of Helicobacter pylori whole cell protein encapsulated by chitosan-alginate
作者：王毅超,邹全明,任建敏,刘健,王晓勤
Author：
收稿日期： 年卷（期）页码：2005,(05):-375-377
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：微球;幽门螺杆菌;壳聚糖;海藻酸钠;表征
Key words：
基金项目：国家“十五”863课题(No.2001AA2151611)
中文摘要
目的以壳聚糖、海藻酸钠为主要材料包裹幽门螺杆菌(HP)全菌超声蛋白抗原,制备新型HP疫苗制剂。方法将海藻酸钠、壳聚糖、大豆油以及HP超声全菌抗原制备成W/O/W型微球。通过扫描电镜、粒径分布仪等检测微球粒径大小;药物溶出度仪、BCA蛋白试剂盒检测微球的蛋白包封率及药物释放速率。结果所制备的微球形态规则,平均粒径3.33μm;蛋白包封率约为64.8%;微球呈显著缓释模式,缓释时间可达6d。结论壳聚糖-海藻酸钠微球包裹全菌蛋白可以作为HP疫苗的新型缓释制剂。
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