尿素酶三甲基壳聚糖亚微球的制备及其相关性质的初步研究
Preparation and characterization of urease N-trimethyl chitosan chloride submicrospheres
作者：陈浮;黄园;
Author：
收稿日期： 年卷（期）页码：2007,(01):-12-14
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：尿素酶;三甲基壳聚糖;亚微球;粒径;包封率
Key words：
基金项目：四川省科技攻关项目(04SG022-009-5)
中文摘要
目的制备尿素酶三甲基壳聚糖亚微球,并对其相关性质进行初步研究。方法采用离子交联法制备尿素酶三甲基壳聚糖亚微球,激光纳米粒度仪测量粒度分布、多分散性和Zeta电位,透射电镜观察粒子的形态,比色法测定酶活性以间接测定包封率,并考察主要影响因素。结果所制备的尿素酶三甲基壳聚糖亚微球形态规则,粒径分布均匀,平均粒径为303.0nm,多分散系数(PD I)=0.175,Zeta电位为18.1 mV;包封率为89.69%。结论采用离子交联法可制备粒度分布均匀、形态规则、具有较高包封率的尿素酶三甲基壳聚糖亚微球。
参考文献
[1]Cinn SJ,Cai A,Nedrud JG,et al.Protection of germfree micefrom infection by Helicobacter felis after active oral or passive IgAimmunization[J].Vaccine,1993,11(4):637-642.
[2]杨淑静.粘膜免疫[J].国外医学免疫学分册,2001,24(6):29.
[3]Thanou MM,Verhoef JC,Junginger HE,Oral drug absorptionenhancement by chitosan and its derivatives[J].Adv Drug DelivRev,2001,52:117-126.
[4]Sieval AB,Thanou MM,Kotz啨AF,et al.Preparation and NMR-characterisation of highly substituted N-trimethyl chitosan chlo-ride[J].Carbohydr Polym,1998,36:157-165.
[5]Thanou MM,Kotz啨AF,Scharringhausen T,et al.Effect of de-gree of quaternisation of N-trimethyl chitosan chloride for en-hanced transport of hydrophilic compounds across intestinal Caco-2 cell monolayers[J].J Control Rel,2000,64:15-25.
[6]MCgee DJ,May CA,Garner RM,et al.Isolation of helicobacterpylori genes that modulate urease activity[J].J Bacteriol,1999,181(8):2477-2484.
[7]刘世伟,孙逊,聂宇,等.载基因壳聚糖纳米粒的制备及其相关性质的初步研究[J].华西药学杂志,2004,19(6):409-411.
【关闭】
