HPLC测定大鼠非索非那定的血药浓度
Determination of Fexofenadine in rat plasma by HPLC
作者：胡玉琴;蒋学华;张岩;王凌;
Author：
收稿日期： 年卷（期）页码：2008,(04):-440-442
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：非索非那定;高效液相色谱法;静脉注射;大鼠血浆浓度
Key words：
基金项目：
中文摘要
目的建立大鼠血浆中非索非那定浓度的测定方法。方法采用HPLC法测定,色谱柱为DikmaTMC18柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.5%磷酸二氢钾(pH3.8)-三乙胺(30:70:0.3),流速1.2 ml.min-1,检测波长220 nm。结果非索非那定0.098~7.000μg.ml-1线性关系良好(r=0.9997,n=7),高、中、低浓度测定的方法回收率为99.1%~104.5%。日内RSD为2.74%~6.67%,日间RSD为3.10%~6.98%。结论所建方法预处理简单?专属性强、灵敏度高,适合测定非索非那定的血药浓度。
参考文献
[1]Markham A,Wagstaff A.Fexofenadine[J].Drugs,1998,55(2):269.
[2]Tsukasa U,Norio YF.Liquid chromatographic determination offexofenadine in human plasma with fluorescence detection[J].JPharm and Biomed Analy,2004,35:937-942.
[3]Hofmann U,Seiler M,Drescher S,et al.Determination of fexofe-nadine in human plasma and urine by liquid chromatography-mass spectrometry[J].J Chromatogr B,2002,766:227-233.
[4]Russell T,Stohz M,Weir S.Pharmacokinetics,Pharmacodynam-ics,and tolerance of single-and multiple-dose fexofenadinehydrochloride in healthy male volunteers[J].Clin Pharmacol T-her,1998,64(6):612-621.
[5]Stohz M,Arumugham T,Iippert C,et al.Effect of food on the bio-availability of fexofenadine hydrochloride(MDL16455A)[J].Biopharm Drug Dispos,1997,18(7):645-648.
[6]Radhakfishna T,Om RG.Simultaneous determination of fexofena-dine and its related compounds by HPLC[J].J Pharm Biomed A-nal,2002,29:681-690.
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