MDMA诱导大鼠神经元凋亡及凋亡相关因子caspase-3的表达
Neuron apoptosis induced by 3,4-methylenedioxy methamphetamine and the expression of caspase-3
作者：王雪;李静;祝三平;
Author：
收稿日期： 年卷（期）页码：2007,(05):-511-513
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：3,4-亚甲基二氧基甲基苯丙胺;神经元凋亡;半胱氨酸天冬氨酸特异性蛋白酶-3;表达
Key words：
基金项目：四川大学华西医院基金资助(专项基金编号:134040162)
中文摘要
目的探讨3,4-亚甲基二氧基甲基苯丙胺(MDMA)对实验大鼠神经元凋亡的诱导及凋亡相关因子半胱氨酸天冬氨酸特异性蛋白酶-3(caspase-3)的表达。方法将20只Wistar雄性大鼠随机均分为1组对照组(A)、3组MDMA实验组(B、C、D)。B组予MDMA(20 mg.kg-1,ip,single),C组予MDMA(20 mg.kg-1,8 am,8 pm,ip×2 d),D组予MDMA(20 mg.kg-1,8 am,8 pm,ip×4 d);A组给予等体积生理盐水。采用TUNEL法检测神经元凋亡,免疫组织化学方法检测Caspase-3的表达。结果给予MDMA后,大鼠各相关脑区有凋亡细胞形成,Caspase-3有不同程度的表达。结论MDMA可导致神经元的凋亡,并诱导凋亡相关因子Caspase-3的表达。
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