HPLC测定拉米夫定中的右旋异构体
Determination of dextroisomer in Lamivudine by HPLC
作者：吴意红;张丹;毛惠英;唐丽红;
Author：
收稿日期： 年卷（期）页码：2009,24(01):-80-81
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：拉米夫定;光学异构体;右旋体;高效液相色谱法;β-环糊精
Key words：
基金项目：
中文摘要
目的采用HPLC法测定拉米夫定中的右旋体。方法采用CyclobondⅠ2000色谱柱(250mm×4.6mm,5μm),甲醇-0.1mol.L-1醋酸铵(5:95)为流动相,流速0.8m.lmin-1,柱温10℃~30℃,检测波长270nm。结果拉米夫定的光学异构体得到了基线分离,其右旋体0.5~5μg.ml-1与峰面积的线性关系良好(r=0.9995),最低检测限为1.5ng。结论所建方法准确、简便、快速,适合于拉米夫定中右旋体的测定。
参考文献
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