南海海绵Iotrochota sp.中抗氧化活性成分的研究
Antioxidant constituents of marine sponge Iotrochota sp. from South China Sea
作者：季红;刘永宏;袁牧;朱柳;黄碧云;
Author：
收稿日期： 年卷（期）页码：2009,24(02):-118-119
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：海绵Iotrochota sp.;Iotrochota属;抗氧化;Purpurone;DPPH自由基;中国南海
Key words：
基金项目：国家自然科学基金项目(批准号:30701051)
中文摘要
目的研究南海海绵Iotrochota sp.中的抗氧化活性成分。方法以95%乙醇浸提得南海海绵总浸膏,经多步分配得乙醇、正己烷和正丁醇部位。采用DPPH法进行活性示踪、反相柱层析和HPLC法分离,光谱方法进行结构鉴定。结果分离得到的化合物鉴定为purpurone,其清除DPPH自由基的IC50为19μg.m l-1。结论Purpurone为海绵Iotrochota sp.的特征性成分和主要抗氧化活性成分,对Iotrochota属海绵的分类具有重要意义。
参考文献
[1]Hooper JNA,Van Soest RWM.Systema Porifera:a guide to theclassification of sponges[M].New York:Kluwer Academic/Plenem Publishers,2002.594-601.
[2]Carletti I,Banaigs B,Amade P.Matemone,a newbioactive bro-mine-containing oxindole alkaloid from the Indian ocean spongeIotrochotapurpurea[J].J Nat Prod,2000,63:981-983.
[3]Deng SZ,Tian CL,Xiao DJ,et al.Iotroridoside-A,a novel cyto-toxic glycosphingolipid from the marine sponge iotrochota ridley[J].中国化学,2001,19(4):362-364.
[4]Chan GW,Francis T,Thureen DR,et al.Purpurone,an inhibitorof ATP-citrate lyase:a novel alkaloid from the marine spongeIotrochotasp.[J].J Org Chem,1993,58:2544-2546.
[5]Brown JW,Kesler CT,Neary JT,et al.Effects of marine spongeextracts on mitogen-activated protein kinase(MAPK/ERK1,2)activity in SW-13 human adrenal carcinoma cells[J].Toxicon,2001,39:1835-1839.
[6]Kumaran A,Karunakaran RJ.Activity-guided isolation andidentification of free radical-scavenging components from anaqueous extract ofColeus aromaticus[J].Food Chem,2007,100(1):356-361.
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