葛根素对大鼠肠缺血再灌注的肠黏膜iNOS基因表达的影响
Effect of puerarin on the expression of iNOS gene in the gut mucosa of intestinal ischemia-reperfusion rats
作者：柳家贤;陈金和;
Author：
收稿日期： 年卷（期）页码：2009,24(03):-247-250
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：缺血再灌注;小肠;葛根素;iNOS
Key words：
基金项目：
中文摘要
目的观察葛根素对肠缺血再灌注大鼠肠黏膜iNOS活性及基因表达的影响。方法♂SD大鼠分为假手术组、肠缺血再灌注2、8 h组、葛根素小、大剂量(70、140 mg.kg-1)+肠缺血再灌注2 h和8 h组、连续ip给药7 d。于第7天给药后30 min麻醉大鼠,复制肠缺血再灌注损伤模型。于再灌注后2、8 h观察大鼠小肠病理形态学的改变并检测血清中一氧化氮(NO)的含量及肠黏膜诱导型一氧化氮合酶(iNOS)的活性,用RT-PCR半定量测定iNOS mRNA的含量。结果大鼠应用葛根素和肠缺血再灌注2 h和8 h后,与模型对照组比较,可减轻肠黏膜的破坏;假手术组大鼠显示较低的黏膜iNOS活性、iNOS mRNA含量及血清NO水平,肠缺血再灌注后则表现出逐渐增加的趋势。至再灌注8 h,模型对照组的iNOS活性、NO水平较假手术组分别增加了64%、75%(P
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