新型靶向FAK基因shRNA的阳离子PCEC纳米材料制剂对人乳腺癌增殖和凋亡的影响
Effect of new nanoparticles which complexed with shRNA targeting FAK on proliferation and apoptosis of human 435S cell
作者：黄安亮;成丽;
Author：
收稿日期： 年卷（期）页码：2009,24(04):-345-348
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：局部粘着斑激酶;阳离子纳米粒;短发卡RNA;增殖;凋亡
Key words：
基金项目：
中文摘要
目的制备PCEC阳离子纳米粒并复合靶向FAK的shRNA的重组质粒,观察其感染人乳腺癌435S细胞所引起的FAK下调对体外肿瘤细胞凋亡的影响。方法用乳液溶剂扩散法制备PCEC阳离子纳米粒;将PCEC阳离子纳米粒与靶向FAK的shRNA的重组质粒静电复合并感染人乳腺癌435S细胞;用Western-bolt检测感染细胞中FAK的下调;通过MTT、PI荧光染色及DNA Ladder分别检测感染细胞的增殖与凋亡情况。结果乳液溶剂扩散法制备出100~200 nm的PCEC阳离子纳米粒;选取100 nm的纳米粒以40:1的质量比与靶向FAK的shRNA的重组质粒复合,感染人乳腺癌435S细胞后,经Western-blot测得FAK蛋白水平明显下调,MTT检测结果为细胞增殖受到明显抑制,经PI染色后细胞、细胞核出现明显的凋亡形态学变化,琼脂糖凝胶电泳显示其基因组DNA有明显的梯状条带(DNA ladder)。结论PCEC阳离子纳米粒复合靶向FAK的shRNA的重组质粒具有抑制435S细胞增殖和诱导凋亡的作用。
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