盐酸维拉帕米PLGA纳米粒的成型工艺及制剂学性质研究
Preparation and characterization of Verapamil hydrochloride loaded PLGA nanoparticles
作者：龚道琼;尹宗宁;宋相容;何治尧;侯世祥;
Author：
收稿日期： 年卷（期）页码：2009,24(06):-583-586
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：维拉帕米;乳酸/羟基乙酸共聚物;纳米粒;乳化-溶剂挥发法
Key words：
基金项目：国家自然科学基金(批准号:30772668);;
863高新技术研究发展计划重点项目(2007AA021810)
中文摘要
目的优化影响盐酸维拉帕米乳酸/羟基乙酸共聚物(PLGA)纳米粒成型工艺的参数,并评价优化工艺后所制纳米粒的制剂学性质。方法采用O/W超声乳化-溶剂挥发法制备盐酸维拉帕米PLGA纳米粒(VRP-PLGANP),以粒径、包封率和载药量为评价指标,采用单因素试验系统考察PLGA浓度、PLGA/VRP质量比、PVA浓度、有机相中丙酮浓度、外水相pH、内外相(O/W)体积比、探头超声时间、旋蒸时间共8个参数对纳米粒成型工艺的作用规律。结果用优化处方工艺制备的纳米粒的包封率和载药量分别为65.78%±6.32%和22.75%±1.48%、平均粒径为150.4±6.9nm、PDI=0.070±0.018(n=3),体外释放规律符合Weibull方程,具有一定的缓释特性。结论所用方法可用于制备载两亲性药物的PLGA纳米粒。
参考文献
[1]Mohammad R,Abbaszadegan,Nils E,et al.Resistance to the Chemo sensitizer Verapamil in a multi-drug-resistan(MDR)human multiple myeloma cell Line[J].Int J Canc,1996,66:506-514.
[2]Rajeev AJ.The manufacturing techniques of various drug loaded biodegradable poly(lactide-co-glycolide)(PLGA)devices[J].Biomaterials,2000,21:2475-2490.
[3]Budhian A,Siegel SJ,Winey KI.Haloperidol-loaded PLGA nan-oparticles:Systematic study of particle size and drug content[J].Int J Pharm,2007,336(2):336-367.
[4]Choi SH,Park TG.G-CSF loaded biodegradable PLGA nanopar-ticles prepared by a single oil-in-water emulsion method[J].Int J Pharm,2006,311(1-2):311-323.
[5]Chong CSW,Cao M,Wong WW,et al.Enhancement of T helper type1immune responses against hepatitis B virus core antigen by PLGA nanoparticle vaccine delivery[J].J Control Rel,2005,102:85-90.
[6]Saxena V,Sadoqi M,Shao J.Indocyanine green-loaded biode-gradable nanoparticles,preparation,physicochemical character-ization and in vitro release[J].Int J Pharm,2004,278:293-301.
[7]Galindo-Rodriguez S,Allémann E,Fessi H,et al.Physico-chemical parameters associated with nanoparticle formation in the salting-out,emulsification diffusion,and nanoprecipitation methods[J].Pharm Res,2004,21:1428-1439.
[8]Liu J,Gong T,Wang C,Zhong Z,et al.Solid lipid nanoparticles loaded with insulin by sodium cholate-phosphatidylcholine-based mixed micelles:preparation and characterization[J].Int J Pharm,2007,340:153-162.
[9]段友容,张志荣,唐永刚,等.mPEG-PLGA-mPEG纳米粒的体外降解规律的研究[J].生物医学工程学杂志,2004,21(6):921-925.
【关闭】
