用培养成熟大鼠头盖骨的成骨细胞筛选药物
NEW DRUG SCREENING USING OSTEOBLAST CLONE CULTIVATED BY ADULT RAT CALVARIS
作者：邓力,郑虎,翁玲玲,井手速雄,桐生道明
Author：
收稿日期： 年卷（期）页码：1998,(02):-85-87
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：成骨细胞;XW630;药物筛选;骨质疏松
Key words：
基金项目：
中文摘要
用周龄在25～35Wistar大鼠的头盖骨，经酶消化处理后，在10％胎牛血清的F－12培养液中培养一周，分别加入地塞米松（Dex）、17β-雌二醇（E2）和新型抗骨质疏松药物XW630，利用相差显微镜动态观察成骨细胞（AOB）骨形成情况，连续观察14～20d，经VonKossa染色，用BioMultiScaner－400（BMS－400ToYoSokki）测定骨结节表面积，作为骨形成定量指标，并作统计学分析，结果表明Dex，XW360有促骨形成作用，E2无促骨形成作用。
参考文献
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