高效液相色谱法测定硝酸异山梨酯注射剂的含量
DETERMINATION OF ISOSORBIDE DINITRATE INJECTION BY HPLC
作者：王磊,邓树海,张延岭,刘澎川
Author：
收稿日期： 年卷（期）页码：1997,(04):-247-248
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：硝酸异山梨酯;注射剂;高效液相色谱法
Key words：
基金项目：
中文摘要
用高效液相色谱法测定了ISDN注射剂的含量。采用NucleosilRpC18色谱柱（250mm×4mm）为固定相，以50％（V／V）甲醇的水溶液为流动相，咖啡因为内标物，检测波长为254um。ISDN的浓度在100～350ng／ml范围内同ISDN／内标峰面积之比成很好的线性关系，回归方程为：Y=-0．0446＋4.63×10－3C，r=0.9999，回收率为100.19±0．25（n＝6）。该法操作简便，测定结果准确可靠。
参考文献
1Shingbal DM.Barad UG.Determination of isosorbide dinitrate and its formulations via colormetric method.Indian Drugs,1985.52(11):607
2中国药典.二部,1990,679
3Torok I. High-performance liquid chromatographic assay and content uniformity determination of nitroglycerin and isosorbide dinitrate in tablets.Acta Pharm Hung.1985,55(5):154
4BP.1988.320
5Nobuyasu M,Chie S.Emiko M.Determination of isosorbide nitrates by high-performance liquid chromatography and their stability in aqueous solution,J, Chromatogr,1983,264:159
6Loren G,Andrew NP.Validation of High-performance liquid chromatographic methods for analysis of sustainedreleslse preparation containing nitroglycerin,isosorbide dinitrate,or pentaergthritrol tetrantrate.J Pharm Sci,1983,72(2):124.
7Dimov N,Agapova N,Levy Sh,et al.Separation of nitrate esters and nitrate-acetate esters of isosorbide.J Chromatogr, 1984.285:515
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