加替沙星对人结肠癌HCT116细胞增殖和周期的影响
Effects of Gatifloxacin on the proliferation and cell cycle of human colon cancer HCT116 cells
作者：王放,陈重华,包旭,李天平
Author：
收稿日期： 年卷（期）页码：2020,35(03):-261-264
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：氟喹诺酮;加替沙星;人结肠癌HCT116细胞;细胞周期;G2期阻滞;细胞凋亡;增殖抑制;抗肿瘤
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基金项目：
中文摘要
目的研究加替沙星(GFX)对人结肠癌HCT116细胞增殖和周期的影响,为研究GFX抗结肠癌的机制和作用靶点提供依据。方法采用CCK8法检测不同浓度(300～500μmol·L~(-1))GFX作用48 h对HCT116细胞增殖的影响;采用PI单染流式细胞技术检测不同浓度GFX作用48 h对HCT116周期的影响;Hoechst33258染色并用荧光显微镜观察GFX对HCT116形态的影响。结果与对照组比较,GFX各浓度组细胞的存活率下降,且呈剂量依赖性;400μmol·L~(-1 )GFX组的G_1期以及300、400μmol·L~(-1 )GFX组的S期细胞的比例均降低,300、400μmol·L~(-1 )GFX组的G_2期细胞的比例显著升高。荧光显微镜下未观察到经GFX处理的细胞存在明显的细胞凋亡特征。结论 GFX能抑制HCT116细胞的增殖并诱发其G_2期周期阻滞。
参考文献
[1] 中国结直肠癌诊疗规范(2017年版)[J].中国实用外科杂志,2018,38(10):1089-1103.
[2] Beberok A,Wrze■ induction of S-phase cell cycle arrest and apoptosis in COLO829 melanoma cells[J].Pharmacol Rep,2018,70(1):6-13.
[3] Gupta P,Gao HL,Ashar Y,et al.Ciprofloxacin enhances the chemosensitivity of cancer cells to ABCB1 substrates[J].Int J Mol Sci,2019,20(2):268.
[4] Song M,Wu H,Wu S,et al.Antibiotic drug levofloxacin inhibits proliferation and induces apoptosis of lung cancer cells through inducing mitochondrial dysfunction and oxidative damage[J].Biomed Pharmacother,2016,84:1137-1143.
[5] Yadav V,Varshney P,Sultana S,et al.Moxifloxacin and ciprofloxacin induces S-phase arrest and augments apoptotic effects of cisplatin in human pancreatic cancer cells via ERK activation[J].BMC Cancer,2015,15(1):581.
[6] Beberok A,Wrze■.Lomefloxacin induces oxidative stress and apoptosis in COLO829 melanoma cells[J].Int J Mol Sci,2017,18(10):2194.
[7] Schneider G,Yadav V,Sultana S,et al.Gatifloxacin induces s and G2-Phase cell cycle arrest in pancreatic cancer cells via p21/p27/p53[J].PLoS ONE,2012,7(10):e47796.
[8] Kan JY,Hsu YL,Chen YH,et al.Gemifloxacin,a fluoroquinolone antimicrobial drug,inhibits migration and invasion of human colon cancer cells[J].Biomed Res Int,2013,2013:1-11.
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