共包载顺铂和二氢卟吩e6脂质体的制备与体外抗肿瘤评价
Preparation of co-loaded cisplatin and chlorin e6 liposomes and the evaluation of the antitumor effects in vitro
作者：徐涛,张米娅,陈亮,曾青,王峥,杨红
Author：
收稿日期： 年卷（期）页码：2020,35(05):-480-486
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：顺铂;二氢卟吩e6;化疗;光动力治疗;联合治疗;薄膜分散法;脂质体;抗肿瘤效果
Key words：
基金项目：
中文摘要
目的制备共包载化疗药顺铂(CDDP)和光敏剂二氢卟吩e6(Ce6)的脂质体(PC-Lip),对其结构和性质进行表征,并在细胞水平上考察其体外抗肿瘤的效果。方法采用薄膜分散法制备PC-Lip,并制备了单包载顺铂及Ce6的脂质体(CDDP-Lip、Ce6-Lip),进行对照,对脂质体的形态、粒径、Zeta电位、包封率及释药行为等进行表征。考察脂质体与4T1细胞的作用,包括脂质体对细胞的毒性、细胞内活性氧(ROS)水平及细胞损伤机制。结果制得的PC-Lip为分散均匀的球形,粒径80.1±2.1 nm,平均水合粒径为128.9±4.1 nm,分散系数
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