miR-17-92簇的表达与小儿急性T淋巴细胞白血病糖皮质激素耐药的相关性研究
Relevant research between the expression of miR-17-92 cluster and pediatric acute T lymphocytic leukemia with glucocorticoid resistance
作者：徐娟娟,徐瑞聆,郭霞,宋相容,付春梅
Author：
收稿日期： 年卷（期）页码：2020,35(04):-381-384
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：小儿急性T淋巴细胞白血病;糖皮质激素耐药;miR-17-92基因簇;荧光定量PCR;表达差异
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基金项目：
中文摘要
目的研究miRNA 17-92簇中各基因在小儿急性T淋巴细胞白血病(T-ALL)糖皮质激素敏感株和耐药株中的表达差异,调控相应miRNA的表达来预测其在小儿T-ALL糖皮质激素耐药中的作用。方法以小儿T-ALL的糖皮质激素耐药株CEM-C1和敏感野生株CEM-C7为研究对象,采用荧光定量PCR技术检测miR-17-92簇中各基因在两株细胞中的表达差异;在CEM-C1中通过瞬时转染技术调控差异表达的miRNA,并检测HES1的变化。结果与CEM-C7比较,CEM-C1中miR-19a、miR-20a和miR-92a的表达均明显上调;下调CEM-C1中的miR-92a后,HES1的表达显著降低。结论 miR-19a、miR-20a和miR-92a在CEM-C1和CEM-C7中存在差异表达; miR-92a可能与小儿T-ALL糖皮质激素耐药密切相关。
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