胰岛素固体脂质纳米粒的制备及其包封率的测定
Preparation of Insulin-loaded solid lipid nanoparticles and determination of encapsulation efficiency
作者：刘洁;张志荣;林芸竹;陈静;吴芸婷;龚涛;
Author：
收稿日期： 年卷（期）页码：2007,(06):-597-599
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：固体脂质纳米粒;胰岛素;高效液相色谱法;包封率;Zeta电位
Key words：
基金项目：国家自然科学基金重点项目(批准号:30430770)
中文摘要
目的制备胰岛素固体脂质纳米粒(Ins-SLNs),考察其理化性质,并建立测定包封率的方法。方法通过复乳/溶剂扩散法制备Ins-SLNs,考察其形态、粒径分布、Zeta电位;通过改变pH,调节Zeta电位后,采用冷冻高速离心分离纳米粒与游离Ins的方法,测定Ins-SLNs的包封率。结果复乳法制备的Ins-SLNs在扫描电镜下均呈球形,分布均匀,平均粒径为114.7±4.68 nm,Zeta电位为-54.36±2.04 mV;包封率测定方法的线性范围为1.047~100.47μg.ml-1,平均回收率为98.37%,RSD=1.02%;测得3批Ins-SLNs样品的平均包封率为97.78%。结论所用制备工艺简单,制得的纳米粒包封率较高;包封率的测定方法方便、灵敏、准确。
英文摘要
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