α-苦瓜素和PEG-α-苦瓜素抑制绒癌JAR细胞迁移和侵袭的研究
Inhibitory effect of α-momorcharin and its PEGylated conjugates on the migration and invasion of human choriocarcinoma JAR cells
作者：陈伊雯;刘敏;姚兴川;王殊睿;孟延发;
Author：
收稿日期： 年卷（期）页码：2011,26(01):-1-5
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：α-苦瓜素;PEG-α-苦瓜素;绒毛膜癌;增殖;迁移和侵袭;金属蛋白酶
Key words：
基金项目：国家自然科学基金(批准号:30770232)
中文摘要
目的考察α-苦瓜素(α-MMC)对绒癌JAR细胞体外迁移侵袭行为的影响及其聚乙二醇(PEG)修饰物保留这一活性的程度。方法利用分子量为20 kDa的聚乙二醇氨基酸衍生物[(mPEG)2-Lys-NHS]以共价连接的方式对α-MMC分子表面进行修饰。采用MTT法、细胞黏附试验、划痕损伤试验、transwell法和明胶酶谱法分别检测JAR细胞经α-MMC或PEG-α-MMC作用后细胞的生长、存活、黏附、迁移、侵袭行为及分泌基质金属蛋白酶(MMPs)能力的变化。结果α-MMC及PEG-α-MMC依赖于剂量和时间方式显著抑制了绒癌JAR细胞的生长,PEG-α-MMC可保留67%天然α-MMC的抗增殖活性(1.5 mg.mL-1作用72 h)。经修饰后蛋白处理,细胞分泌的基质金属蛋白酶-2(MMPs-2)活性减弱,削弱了细胞的运动能力,JAR细胞的行进过程受到抑制。结论α-MMC及其PEG修饰物通过减弱MMPs-2活性抑制绒癌细胞的体外行进过程,首次揭示了核糖体失活蛋白可能影响体外肿瘤转移过程。
英文摘要
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