氨基葡萄糖功能化量子点的制备与表征
Preparation and characterizations of glucosamine modified quantum dots
作者：王月静;周许;龚涛;张志荣;符垚;
Author：
收稿日期： 年卷（期）页码：2018,33(04):-343-345
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：氨基葡萄糖;量子点;葡萄糖转运蛋白2;肾小管上皮细胞;合成;表征;紫外吸收光谱;细胞摄取
Key words：
基金项目：国家自然科学基金资助项目(批准号:81503018)
中文摘要
目的制备氨基葡萄糖修饰的量子点(QDs-GlcN),并初步考察其体外性质。方法利用氨基葡萄糖的氨基与量子点表面的羧基酰化反应制备QDs-GlcN,考察其电位、粒径、荧光发射和紫外吸收光谱、细胞的毒性及细胞摄取情况。结果QDs和QDs-GlcN的Zeta电位分别为-30.4、-26.2 mV;QDs和QDs-GlcN呈类球形,粒径的分布均一,平均粒径约10 nm;QDs和QDs-GlcN的紫外吸收光谱和荧光发射光谱无显著性差异;0~4 nmol·L~(-1)的QDs和QDs-GlcN对大鼠肾小管上皮细胞NRK52E未见明显毒性,且QDs-GlcN在NRK52E细胞的摄取效率显著高于QDs。结论 QDs-GlcN细胞的相容性良好,氨基葡萄糖的修饰可显著提高靶细胞对量子点的摄取效率,可为肾病的靶向诊断及治疗提供参考。
英文摘要
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