人类牙乳头细胞的体外培养及细胞生物学性状研究

Objective To culture human dental papilla cells(HDPCs)and to study its cytobiological charactersin vitro.Meth- ods HDPCswere isolated and cultured with explant culture techniquein vitro; TypeⅠcollagen, fibronection and lamininwere detected in HDPCs and its secreted matrix with the immunocyto-chemical stain; HDPCs were incubated in mineralized promoting solution containing 10 mmol/Lβ-glycerophosphate, 100 mg/L of ascorbic acid and 10 nmol/L dexamethasone supplemented with 10% FBS and the form of mineralized nodules was tested with Alizarin Red S stainning.Results Cultured HDPCsin vitrowere well growing in DMEM/F12. TypeⅠcollagen, fibronection and laminin stainingwere all positive in bothHDPCs and its secreted matrix, and laminin was stained with bunchiness in matrix. Mineralized nodules formed after cultured 27 days by Alizarin Red S stainning.Conclusion HDPCs isolated and cultured are well growingin vitro, have a capability of synthesizing and secreting matrix and in mineralized promoting solution, are able to formmineralizer, so, HDPCs have a capacity of seed cell of tissue engi- neering regeneration tooth.