组织工程化口腔黏膜移植修复裸露硬腭对上颌骨生长发育影响的研究

Objective To observe the effectiveness of prevention and cure for maxillary growth deformity following tissue engineered oral mucosa implantation on mucoperiosteal denuded palate process in young rat.Methods Hard palate mucoperios- teum of a SD baby ratwere excised and oral keratinocyteswere isolated and cultured. Tissue engineered oral mucosawas fabricated with the cultured oral keratinocytes and the membrane made of sodium alginate (SA). 80 female three-week-old SD ratswere used as subjects in this study. The animals were divided randomly into a normal control group and 3 experimental groups, each group included 20 rats. Normal control group (NG) were not operated. Hard palate mucoperiosteum on left side in all experimental groups were excised, exposed bonewere nottreated in denuded group (DG), but repairedwithmembrane inmaterial group (MG) and repaired with the tissue engineered oral mucosa in mucosal group (MUG). All the animalswere sacrificed at 9thweek postop- eratively (12 weeks old), and the clean widths of right and left hard palatal were measured under a dissection microscope. The difference between palatal widths of two sides and the asymmetry ratio between the different groups were compared and analyzed. Results No significant difference in asymmetrywas discovered between the DG and theMG, butthe asymmetry inMUGwas less than DG orMG.Conclusion Tissue engineered oral mucosal implantation in palatoplasty is an effective method in preventing and curing secondary maxilla deformity by repairing denuded bone wound.