Objective To study the relationship between endothelial nitric oxide synthase（eNOS） Glu298Asp gene polymorphism and chronic periodontitis（CP） with type 2 diabetes mellitus（T2DM）. Methods DNA from patients’buccal swabs of CP, CP with T2DM, T2DM and health was isolated and extracted. The eNOS Glu298Asp gene polymorphism were assessed by polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP） respectively. Results The distribution of eNOS Glu298Asp genotype in CP group, T2DM group, CP with T2DM group and health group showed statistical differences（掊2＝18.503, P＝0.005）, and the gene frequency showed statistical differences（掊2= 8.243, P=0.041）. Compared health group to CP with T2DM group, OR value of the genetype T was 0.962, 95% confidence interval lay in between 0.737 to 1.256, which showed T maybe a protective factor. While OR value of the genetype G was 1.043, 95% confidence interval lay in between 0.781 to 1.391, which showed G maybe a risk factor. However, neither T nor G was statistically significant. Conclusion Based on these findings, there are some association between eNOS Glu298Asp polymorphism and the risk of CP group, T2DM group, CP with T2DM group.
