高迁移率族蛋白1在慢性牙周炎病变牙龈组织中的表达
Expression of high mobility group box 1 in gingival tissues of chronic periodontitis
作者：赵华强 穆萍萍 魏玲玲 侯萌 孙钦峰 宋晖 杨丕山
Author：Zhao Huaqiang, Mu Pingping, Wei Lingling, Hou Meng, Sun Qinfeng, Song Hui, Yang Pishan
收稿日期： 年卷（期）页码：2013,31(2):191-191-194
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：高迁移率族蛋白,牙周炎,龈沟液,外周血单核细胞,
Key words：high mobility group box 1,periodontitis,gingival crevicular fluids,peripheral blood mononuclear cell,
基金项目：
山东省自然科学基金资助项目（Y2008C99）；山东省优秀中青年科学家奖励基金资助项目（BS2009SW057）
中文摘要
目的 研究慢性牙周炎病变牙龈组织中高迁移率族蛋白1（HMGB1）的表达。方法 提取健康志愿者外周血单核细胞（PBMC），以1 pg·mL-1的细菌脂多糖（LPS）刺激细胞，24 h后用免疫荧光染色法检测HMGB1的表达，48 h 后用酶联免疫吸附试验检测细胞上清液中HMGB1的表达；分别以50 ng·mL-1肿瘤坏死因子-α（TNF-α）和100 ng? mL-1 HMGB1刺激PBMC，48 h后检测细胞上清液中HMGB1和TNF-α的表达。另外收集健康者和慢性牙周炎患者的牙龈组织和龈沟液，分别检测牙龈组织和龈沟液内HMGB1的表达。结果 LPS刺激PBMC 24 h后，HMGB1自细胞核移出至细胞质中；刺激48 h后，细胞上清液中HMGB1的表达量明显高于对照组（P结论 HMGB1可能在牙周炎病理进程中有重要作用。
英文摘要
Objective To investigate the expression of high mobility group box 1 (HMGB1) in gingival tissues of chronic periodontitis.Methods Human peripheral blood mononuclear cells (PBMC) were stimulated with 1 jxg ? mL-1 lipopolysaccharide (LPS) for 24 h or 48 h. Expression and release of HMGB1 were checked by immunofluorescence and enzyme -linked immunosorbent assay (ELISA) , respectively. PBMC were stimulated with 100 ng·mL-1 HMGB1 or 50 ng ? mL-1 tumor necrosis factor-a (TNF-α) , the expressions of TNF-α and HMGB1 in the supernatant were studied by ELISA. Gingival tissues and gingival crevicular fluids (GCF) were collected from patients and healthy people. Expression of HMGB1 in gingival tissues and GCF was studied using immunofluorescence and ELISA, respectively.Results HMGB1 was translocated from nucleus to cytosol in PBMC after LPS stimulation for 24 h. The content of HMGB1 in the supernatant from stimulated cells was significantly higher than that from unstimulated cells after 48 h (PConclusion The results suggest that HMGB1 may play an important role in the pathological progress of chronic periodontitis.
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