分泌型卷曲相关蛋白1与慢性牙周炎的相关分析
Exploring the relationship between secreted frizzled-related protein 1 and chronic periodontitis
作者：袁海波 金晶 许春姣 邱喜丽 王璐 吕逢源 刘婷婷 吴颖芳 尹晓敏 方厂云 阙国鹰 彭解英
Author：Yuan Haibo, Jin Jing, Xu Chunjiao, Qiu Xili, Wang Lu, Lü Fengyuan, Liu Tingting, Wu Yingfang, Yin Xiaomin, Fang Changyun, Que Guoying, Peng Jieying
收稿日期： 年卷（期）页码：2013,31(6):615-615-618
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：慢性牙周炎,龈沟液,分泌型卷曲相关蛋白1,
Key words：chronic periodontitis,gingival crevicular fluid,secreted frizzled-related protein 1,
基金项目：
湖南省科技厅基金资助项目（2011SK3254）
中文摘要
目的 检测分泌型卷曲相关蛋白1（SFRP1）在慢性牙周炎（CP）患者和牙周健康者的表达情况，探讨SFRP1在CP的发生与发展过程中的作用。方法 选择CP患者28例为试验组，按临床附着丧失（CAL）程度分为轻、中、重度3组，另外选择牙周健康者10例为对照组。收集两组研究对象的龈沟液，采用酶联免疫吸附法测定龈沟液中SFRP1的质量浓度。取试验组中22例CP患者的病变牙龈组织标本，以及10例对照组的正常牙龈组织，进行SFRP1免疫组织化学染色，观察着色强度，分析SFRP1表达与不同程度CP的相关性。结果 1）试验组和对照组龈沟液中SFRP1的质量浓度分别为（281.07±33.37）和（245.30±35.69）ng·L-1，试验组明显高于对照组（P0.05）。结论 SFRP1在CP患者龈沟液及牙龈组织中的表达高于牙周健康者，SFRP1可能参与了CP的发生发展过程。
英文摘要
Objective This study detects the expression of secreted frizzled-related protein 1 (SFRP1) in healthy patients and patients with chronic periodontitis (CP) and explores the relationship between SFRP1 and the occurrence and development of CP.Methods First, 28 patients forming the CP group were further divided into mild, moderate, and severe CP subgroups according to clinical attachment loss (CAL) data. Ten healthy volunteers were recruited in the control group. Gingival crevi-cular fluid (GCF) was collected from all of the patients, and the concentration of SFRP1 in the GCF samples was detected using enzyme-linked immunosorbent assay. Next, gingival lesions were obtained from 22 patients in the CP group and healthy gingival tissues were obtained from the 10 healthy patients in the control group. Immunohistochemical analysis for SFRP1 was used to analyze the correlation between the expression of SFRP1 and the severity of CP based on staining intensities.Results The concentration of SFRP1 in GCF samples taken from of the CP group (281.07 ng·L-1±33.37 ng·L-1) was signifi-cantly higher than that in samples taken from the control group (245.30 ng·L-1±35.69 ng·L-1) (P0.05).Conclusion SFRP1 expression in the CP groups was significantly higher than that in the control group. Thus, SFRP1 may play a significant role in the development of CP.
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