三氧化矿物凝聚体对乳、恒牙牙髓细胞增殖和分化影响的比较
Effects of mineral trioxide aggregate on the proliferation and differentiation of human pulp cells from primary and permanent teeth
作者：王敏永1 刘鹤1 李盛林2 秦满1
Author：Wang Minyong1, Liu He1, Li Shenglin2, Qin Man1
收稿日期： 年卷（期）页码：2015,33(1):75-75-79
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字： ,三氧化矿物凝聚体,氢氧化钙,碱性磷酸酶,牙本质涎磷蛋白,骨保护因子,核因子&kappa,B受体活化因子配体,牙髓细胞,
Key words：mineral trioxide aggregate,calcium hydroxide, alkaline phosphatase, dentin sialophosphoprotein, osteoprotegerin, receptor activator of NF-κB ligand,dental pulp cell,
基金项目：
中文摘要
目的 对比三氧化矿物凝聚体（MTA）和氢氧化钙对人乳、恒牙牙髓细胞增殖和分化的影响。方法 采用甲基噻唑基四唑（MTT）法检测牙髓细胞生长增殖变化；实时荧光定量聚合酶链反应（PCR）检测碱性磷酸酶（ALP）、牙本质涎磷蛋白（DSPP）、骨保护因子（OPG）、核因子κB受体活化因子配体（RANKL）基因的表达。结果 氢氧化钙组乳、恒牙牙髓细胞增殖均显著低于对照组（P0.05）。恒牙牙髓细胞氢氧化钙组ALP和DSPP的表达与对照组相比无显著差异（P>0.05），MTA组ALP和DSPP的表达显著增加（P0.05）。结论 MTA比氢氧化钙更适合做乳牙和恒牙的盖髓剂，其优势在乳牙可能更为明显。
英文摘要
Objective This study aims to investigate the effects of mineral trioxide aggregate (MTA) and calcium hydroxide on proliferation and differentiation of human pulp cells from primary and permanent teeth.Methods Cell proliferation was detected by methyl thiazolyl tetrazolium (MTT) assay. The mRNA expression levels of dentinogenesis-related factors, including alkaline phosphatase (ALP) and dentin sialophosphoprotein (DSPP), and odontoclastogenesis-related factors, such as osteoprotegerin (OPG) and receptor activator of NF-κB ligand (RANKL), were determined by real time polymerase chain reaction (PCR).ResultsPrimary and permanent pulp cells treated with calcium hydroxide exhibited significantly lower proliferation rates than the control cells (P0.05). Likewise, MTA-treated permanent pulp cells showed significantly upregulated ALP and DSPP expression (P0.05). Neither MTA nor calcium hydroxide affected OPG and RANKL expression in permanent pulp cells (P>0.05).Conclusion MTA is more suitable as a pulp-capping agent, particularly in primary teeth, than calcium hydroxide.
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