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论文摘要

热疗对人Tca8113细胞侵袭能力及基质金属蛋白酶-13和钙离子结合蛋白S100A4表达的抑制作用

The invasion ability and expressions of matrix metalloproteinase-13 and calcium-binding protein S100A4 are inhibited by hyperthermia in human Tca8113 cells

作者:何丽明 边莉 唐睿珠 何永文

Author:He Liming1, Bian Li2, Tang Ruizhu3, He Yongwen4

收稿日期:2011-12-25          年卷(期)页码:2011,29(06):655-655-659

期刊名称:华西口腔医学杂志

Journal Name:West China Journal of Stomatology

关键字:热疗,Tca8113细胞,侵袭,基质金属蛋白酶-13,钙离子结合蛋白S100A4,

Key words:hyperthermia,Tca8113 cells,invasion,matrix metalloproteinase-13,calcium-binding protein S100A4,

基金项目:

国家自然科学基金资助项目(30960422,81160325);云南省自然科学基金资助项目(2009CC021)

中文摘要

目的研究热疗对Tca8113细胞的侵袭能力及侵袭转移相关蛋白———基质金属蛋白酶-13(MMP-13)及钙离子结合蛋白S100A4(S100A4)表达的影响。方法Tca8113细胞分别经43℃加热0、40、80、120 min及37、41、43、45℃加热80 min,用体外侵袭实验评价各组细胞的侵袭能力;Tca8113细胞爬片经43 ℃分别加热0、40、80、120 min,用免疫细胞化学染色法检测各组细胞MMP-13及S100A4的表达变化;Tca8113细胞分别经43℃加热0、40、80、120 min及37、41、43、45 ℃加热80 min,Western blot法检测各组细胞MMP-13及S100A4的表达变化。结果随加热时间延长或加热温度升高,Tca8113细胞侵袭率(即侵袭细胞数占全部细胞的百分比)依次递减,除43 ℃加热40、80 min组之间及41、43℃加热80 min组之间的差异无统计学意义(P>0.05)外,其余各组间的差异均有统计学意义(P

英文摘要

Objective To study the effect of hyperthermia on anti-invasion of Tca8113 and the expression change of matrix metalloproteinase-13(MMP-13) and calcium-binding protein S100A4(S100A4). Methods Tca8113 cell pools were incubated at 43℃ for 0, 40, 80, 120 min, respectively, and at 37, 41, 43, 45 ℃ respectively for 80 min. The effect of high temperatures on the invasion ability of Tca8113 was measured in vitro. The slides of cells were made and incubated at 43 ℃ for 0, 40, 80, 120 min, respectively. Immunocytochemical method was employed for detecting the expression change of MMP-13 and S100A4 protein. Tca8113 cells were incubated at 43 ℃ for 0, 40, 80, 120 min respectively and at 37, 41, 43, 45 ℃ respectively for 80 min. Western blot method was conducted for detecting the expressionchange of MMP-13 and S100A4 protein. Results As incubating time at higher temperature lasted, the proportion of the cells with invasion ability decreased. Except groups of 40 min and 80 min at 43 ℃ and 41, 43 ℃ for 80 min, the rest groups show significant statistic differences(P

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