酸性微环境对单核/巨噬细胞分泌血管内皮细胞生长因子及Tca8113细胞杀伤作用的影响
Experimental study on the anti-tumor effect of monocytes/macrophages against Tca8113 cells and the secretion of vascular endothelial growth factor in acid microenvironment
作者：宋宇峰 冯红超 唐路 彭江帆 马洪
Author：SONG Yu-feng, FENG Hong-chao, TANG Lu, PENG Jiang-fan, MA Hong
收稿日期：2010-08-25 年卷（期）页码：2010,28(04):364-364-366
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：单核/巨噬细胞,舌鳞状细胞癌,酸性,血管内皮细胞生长因子,
Key words：monocytes/mcrophages,tongue squamous cell carcinoma,acid,vascular endothelial growth factor,
基金项目：
国家自然科学基金资助项目（30660200）；贵州省优秀科技教育人才省长专项基金资助项目[（2005）160号]
中文摘要
目的通过酸性微环境下单核/巨噬细胞分泌血管内皮细胞生长因子（VEGF）及对舌癌细胞株Tca8113杀伤作用的研究，探讨肿瘤内浸润的单核/巨噬细胞在酸性微环境影响下的功能状态。方法从健康人的外周血中分离单核细胞并通过培养将其转化为单核/巨噬细胞，分别调整培养液的pH值为酸性：6.6、6.8；常规环境：pH值为7.2，将单核/巨噬细胞和舌癌细胞株Tca8113进行混合培养，MTT法检测单核/巨噬细胞对舌癌细胞的杀伤作用；采用ELISA法检测不同微环境下单核/巨噬细胞VEGF的分泌情况。结果在酸性微环境下，单核/巨噬细胞对舌癌细胞株的杀伤作用低于常规环境下（P
英文摘要
Objective To investigate the anti-tumor effect of monocytes/macrophages against Tca8113 cells and the secretion of vascular endothelial growth factor（VEGF） in acid microenvironment in vitro. Methods Peripheral blood mononuclear cells were extracted from healthy person′ s blood and cultivated to transform into monocytes/ macrophages. The monocytes/macrophages were cultured with Tca8113 in acid microenvironment（pH6.6 and pH6.8） and in normal microenvironment（pH7.2）. The anti-tumor effect of monocytes/macrophages against Tca8113 cells were examined by MTT assay. The expression of VEGF was detected by enzyme-link immunoassay（ELISA）. Results The anti -tumor effect of monocytes/macrophages against Tca8113 cells in acid environment was lower than in normal environment（P
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