期刊导航

论文摘要

骨碎补柚皮苷对人牙周膜细胞总蛋白合成及超微结构影响的实验研究

Effect of Drynaria fortunei naringin on the total protein content and ultra-structure of human periodontal ligament cells

作者:蒋俊强 蔡炜 王忠朝 丁一 李小玉

Author:JIANG Jun-qiang1, CAI Wei1, WANG Zhong-chao1, DING Yi2, LI Xiao-yu3

收稿日期:2010-06-25          年卷(期)页码:2010,28(03):330-330-333

期刊名称:华西口腔医学杂志

Journal Name:West China Journal of Stomatology

关键字:骨碎补,柚皮苷,牙周膜细胞,

Key words:Drynaria fortunei,naringin,periodontal ligament cells,

基金项目:

四川省卫生厅科研基金资助项目[川卫办发(2007)431号]

中文摘要

目的研究骨碎补柚皮苷对人牙周膜细胞总蛋白合成、超微结构的影响。方法采用酶消化法结合组织块法,体外培养人牙周膜细胞;采用考马斯亮蓝染色法,测定不同质量浓度骨碎补柚皮苷对人牙周膜细胞作用不同时间后,细胞内总蛋白质量浓度的变化;采用透射电镜观察骨碎补柚皮苷作用后人牙周膜细胞超微结构的变化。结果100、10、1、0.1 mg·L-1 4种质量浓度的骨碎补柚皮苷在第3、5、7天均能促进人牙周膜细胞内总蛋白的合成,其中以1 mg·L-1组促进作用最强。骨碎补柚皮苷作用于人牙周膜细胞后,细胞质内线粒体、粗面内质网、核糖体明显增多。结论骨碎补柚皮苷可促进人牙周膜细胞蛋白质合成,使细胞代谢更旺盛,功能更活跃。

英文摘要

Objective To evaluate the effects of Drynaria fortunei naringin on the total protein content and ultra- structure of human periodontal ligament cells(hPDLCs). Methods Through enzyme digestion combined tissueculture method, primarily culture and identify the human periodontal ligament cells. Coomassie brilliant blue staining was used to detect the total protein content of hPDLCs with the effects of difference concentration of Drynaria fortunei naringin at difference times. Transmission electron microscope was used to study the ultra-structure of hPDLCs with the effects of Drynaria fortunei naringin. Results In vitro, the addition of Drynaria fortunei naringin at dose of 100, 10, 1, 0.1 mg·L-1 in cultures resulted in an increase of total protein content at the 3rd, 5th, 7th day, but the maximum response was obtained with 1 mg·L-1 Drynaria fortunei naringin. There were more rough endoplasmic reticulums, mitochodrias and ribosomes in the experimental group than in the control. Conclusion Drynaria fortunei naringin may stimulate the protein synthesis and metabolism of hPDLCs.

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