超声冲洗对感染根管内毒素的灭活及清除作用
Study on the effect of ultrasonic irrigation in deactivating and eliminating the endotoxin in the root canals of chronic periapical periodontitis
作者：刘桂香 王青 窦慧芹
Author：LIU Gui-xiang1，2, WANG Qing1, DOU Hui-qin2
收稿日期：2010-04-25 年卷（期）页码：2010,28(02):158-158-161
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：慢性根尖周炎,根管预备,超声冲洗,内毒素,动态浊度法鲎试验,
Key words：chronic periapical periodontitis,root canal preparation,ultrasonic irrigation,endotoxin,kinetic turbidimetric limulus test,
基金项目：
山东省自然科学基金资助项目（Y2006C48）；山东省卫生厅基金资助项目（2001CALCAB15）
中文摘要
目的探讨超声冲洗对感染根管内毒素的灭活及清除作用。方法选择因严重慢性根尖周炎拔除的具有双根管的离体上颌前磨牙10颗，常规开髓，用改良双敞法预备根管，将3％过氧化氢溶液30 μL注入根管，行超声振荡，每根管振荡2 min，分别于根管预备后和超声振荡后取样，测量内毒素活性。另外在临床患者中选择患有慢性根尖周炎的双根管上颌前磨牙10颗，采用相同的方法进行根管预备，预备后以无菌蒸馏水为介质进行超声冲洗，每根管均冲洗2 min，分别于根管预备前、根管预备后和超声冲洗2 min后根管取样，测量内毒素活性。内毒素活性的测定采用动态浊度法鲎试验，并对超声处理前后根管内毒素活性的差异进行分析。结果离体牙超声振荡前，根管内毒素的活性平均为4.069 EU·mL-1，超声振荡后为16.410 EU·mL-1，二者差异有统计学意义（P
英文摘要
Objective To study the effect of ultrasonic irrigation in deactivating and eliminating the endotoxin in the root canals of chronic periapical periodontitis. Methods 10 maxillary premolars with 2 root canals extracted because of serious chronic periapical periodontitis were opened conventionally and prepared with modified double-flared technique with apical foramen sealed. 30 μL 3% H2O2 solution was introduced into each root canal and 2 minutes ultrasonic vibration was given. Samples were taken before and after ultrasonic vibration. 10 maxillary premolars with 2 root canals of chronic periapical periodontitis of 10 patients in clinic were selected. The root canals of them were instrumented as above, followed by 2 minutes ultrasonic irrigation with asepsis distilled water. Samples were taken before, after root canal preparation and after ultrasonic irrigation. The endotoxin activity of them was tested by kinetic turbidimetric limulus test. Results The mean endotoxin activity of extracted premolars before ultrasonic vibration was 4.069 EU·mL-1 while after ultrasonic vibration it was 16.410 EU·mL-1. There was great statistical difference between them（P
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