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论文摘要

重组人白细胞介素-1β诱导人牙髓细胞蛋白质组的差异分析

Differential proteomics analysis of dental pulp cell induced by recombinant human interleukin -1β

作者:郭世梁 张颖丽 黄洋

Author:GUO Shi -liang1, ZHANG Ying -li1, HUANG Yang2

收稿日期:2009-10-25          年卷(期)页码:2009,27(05):487-487-491

期刊名称:华西口腔医学杂志

Journal Name:West China Journal of Stomatology

关键字:重组人白细胞介素-1β,双向电泳,蛋白质组,牙髓细胞,肽质量指纹技术,

Key words:recombinant human interleukin-1β,two-dimensional electrophoresis,proteome,dental pulp cells,peptide mass fingerprint,

基金项目:

吉林省科技厅基金资助项目(200905178)

中文摘要

目的分析重组人白细胞介素-1β(rhIL-1β)诱导前后牙髓细胞蛋白质的差异,鉴定2组间差异表达的蛋白质。方法采用双向凝胶电泳技术分离牙髓细胞全蛋白,获得对照组和诱导组牙髓细胞蛋白质图谱,Image Master2D Elit 5.0软件分析确认差异表达蛋白。通过基质辅助的激光解吸飞行时间质谱对差异表达的蛋白质点进行质谱鉴定,得到肽质量指纹图谱。结果比较对照组和诱导组蛋白质图谱,发现了39个蛋白质点差异明显。其中15个蛋白质点在诱导组高表达,新增13个蛋白质点,7个蛋白质点低表达,4个蛋白质点仅在对照组中表达;质谱鉴定后,10个蛋白得到确认。结论牙髓细胞对rhIL-1β的应答反应是一个非常复杂的过程,多种蛋白质分子涉及其中。鉴定了牙髓细胞中与rhIL-1β作用密切相关的2个差异蛋白,为探索早期牙髓炎的应答机制提供了新的线索和思路。

英文摘要

Objective To compare the proteomics change of human dental pulp cells induced by recombinant human interleukin -1β(rhIL -1β). Methods The dental pulp cell entire protein was separated by a two -dimensional electrophoresis(2-DE) technique. The rhIL-1β induction and the normal dental pulp cell protein 2-DE atlas were established. Difference expression protein was confirmed by ImageMaster 2D Elite 5.0 software analysis. To identify differentially expressed proteins spot by matrix -assisted laser desorption/ionization time of flight mass spectrometry, and get peptide mass fingerprinting. Results Comparing the two groups of protein 2 -DE atlas, 39 protein spots were obviously different. Including 15 points in the induction of protein expression were higher, 13 new protein spots, 7 protein points expressions were lower, there were only four points in the control group. After mass spectra identification, 10 protein spots were confirmed at last. Conclusion Pulp cells to rhIL-1β responsiveness is a very complex process, which involve a variety of protein molecules. rhIL-1β related 10 protein spots have been identified in the dental pulp cell for the first time. To explore pulpitis′s early response mechanism provides a new clue and ideas.

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