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论文摘要

β-连环蛋白和腺瘤性结肠息肉病蛋白在小鼠牙胚发育中的表达及相互关系的研究

Expression of β -catenin and adenomatous polyposis coli protein and correlation between them in the development of mouse tooth germ

作者:姜瑞中 朱恩新 刘婷娇

Author:JIANG Rui-zhong1, ZHU En-xin2, LIU Ting-jiao2

收稿日期:2009-08-25          年卷(期)页码:2009,27(04):370-

期刊名称:华西口腔医学杂志

Journal Name:West China Journal of Stomatology

关键字:β-连环蛋白,腺瘤性结肠息肉病蛋白,牙胚,免疫组化,

Key words:β-catenin,adenomatous polyposis coli protein,tooth germ,immunohistochemistry,

基金项目:

国家自然科学基金资助项目(30700955/c03031102)

中文摘要

目的观察β-连环蛋白(β-catenin)和腺瘤性结肠息肉病(APC)蛋白在胎鼠下颌第一磨牙牙胚中的表达,探讨两者的作用及相互关系。方法将昆明小鼠按雌雄比2∶1合笼,发现阴栓视为妊娠,定为E0.5 d,制作E13.5 d、E14.5 d、E15.5 d、E16.5 d、E17.5 d胎鼠下颌第一磨牙石蜡切片,免疫组化SP法检测β-catenin和APC蛋白的表达与分布。结果β-catenin在牙胚蕾状期、帽状期、钟状期上皮内均有表达,且随着发育的成熟,其表达逐渐增强,着色于细胞膜、质及核。APC蛋白在蕾状期牙蕾上皮呈强阳性表达,随着牙胚发育其表达逐渐减弱,着色在细胞膜、质。β-catenin与APC蛋白有显著的负相关性(P

英文摘要

Objective To examine the distributions of β-catenin and adenomatous polyposis coli(APC) protein in the tooth germ, and obtain the messages of function of the two factors and the relationship between them. Methods Mice were selected and cohabited with the ratio of female mice to male ones being 2∶1, and Embryo day 0.5 was confirmed based on the finding of vaginal plug. The distributions of β-catenin and APC protein in the Embryos on day 13.5, 14.5, 15.5, 16.5, 17.5 were examined in the paraffin-embedded sections by immunohistochemistry methods. Results During E13.5 d to E17.5 d, positive expression of β-catenin was found in the oral epithelium and the dental lamina, and became more and more strong. The staining were whole cell. During the bud stage, strong positive expression of APC protein was found in the oral epithelium and the dental lamina, but the expression displayed a down -regulation tendency. The staining was the cytomembrane and cytoplasm. There was negative correlation between β-catenin and APC protein(P

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